A phosphate-buffered saline answer serves as a basic part in circulation cytometry. This isotonic answer, sometimes composed of sodium chloride, sodium phosphate, and, in some formulations, potassium chloride and potassium phosphate, maintains a secure pH and osmolarity, mimicking physiological circumstances. Its main function is to droop cells or different organic samples throughout the evaluation course of, guaranteeing that cells stay viable and retain their native properties. As an illustration, cells stained with fluorescent antibodies are sometimes washed and resuspended inside this answer previous to their introduction into the circulation cytometer.
The importance of this buffered answer stems from its skill to attenuate cell harm and aggregation, each of which may negatively influence the accuracy and reliability of circulation cytometry knowledge. By offering a secure and appropriate atmosphere, it prevents osmotic shock and preserves mobile integrity. Its use dates again to the early improvement of circulation cytometry and has develop into a standardized observe, contributing considerably to the reproducibility and robustness of experimental outcomes. Its well-defined composition and buffering capability make it a useful reagent in cell-based assays.
